Hyo Joo Kim, Gonzalez Escall and Shanshan Feng were one shot back at 65, followed another shot back by Alena Sharp, Emily Pedersen, and Jodi Ewart Shadoff.
Reavie started the day two shots off the lead. "I need to just keep doing the same thing I'm doing: attack the holes that suit my eye and make as many birdies as I can".
The American, who qualified for next week's U.S. Open, is alongside Matt Every, Scott Brown and Sebastian Munoz atop the leaderboard.
He sank birdie putts from about 10 feet away at both the second and sixth, and sank a shorter birdie attempt at the ninth.
Starting on No. 10, Mickelson made three birdies and no bogeys on his opening nine. "It's just - I feel like I wasn't even on the golf course".
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"I'm one-for-one defending titles, so that's a pretty good stat", said Berger, who claimed his first and previously only PGA tour victory at last year's St Jude Classic. After one round in Iowa, he's tied with two golfers who've never won on the senior circuit.
Fowler is 10 shots off the lead, shared by four men on six under.
The 2015 PGA Tour Rookie of the Year earned his first Tour win at the same showcase a year ago to find some momentum. "It was as if I'd never won before, as if I was a rookie". "So going back to 2008 probably, the first half of '08, and I really, really felt good about my game and I felt like I had a chance to win a lot of tournaments".
Every went through bogey-free, an eagle at the par-five 16th the highlight of his round.
Calcavecchia made himself a fan favorite two years ago by wearing bacon-themed trousers in a state famous for its pork production.
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Phil Mickelson briefly had a share of the lead but had triple bogey on No. 12, including a shot into the water.
Reavie said he'd stay aggressive over the weekend.
Fowler, the world number nine, battled as he shot a four-over 74.
Jaco Van Zyl, Johan Carlsson, and Ashley Chesters shot 67 to share second, with Graeme Storm, Oliver Wilson, and Joel Stalter one stroke further behind in fifth.
Defending champion Wu Ashun of China and 2012 victor Bernd Wiesberger each shot a two-under 70. The Austrian, the highest-ranked player in the field at 29th, completed his round with two straight birdies.
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Jodi Ewart Shadoff of England (69) was one shot back in fourth place.
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